L-82Homo sapiens (Human)Cancer cell line
Also known as: L82
🤖 AI SummaryBased on 2 publications
Quick Overview
Human ALCL cell line for cancer research and therapy studies.
Detailed Summary
L-82 is a human anaplastic large cell lymphoma (ALCL) cell line established from the pleural effusion of a 24-year-old patient with recurrent ALCL. The cell line exhibits typical morphological features of ALCL, including irregular nuclear profiles, prominent nucleoli, and abundant cytoplasm. Immunophenotyping shows positivity for CD30, EMA, CD34, CD42, CD57, TI1A1, and granzyme B, with negativity for EBV-related antigens. Cytogenetic analysis reveals a complex, near-triploid karyotype with 72-77 chromosomes, including the ALCL-specific translocation t(2;5)(p23;q35). L-82 cells form tumors in SCID mice, making them a valuable model for studying ALCL biology and testing therapeutic strategies. The cell line also shows a Th2 cytokine profile, suggesting potential roles in immune modulation and tumor growth.
Research Applications
Cancer researchTherapy developmentImmunotherapy studiesGenetic and molecular studies
Key Characteristics
CD30 positivet(2;5) translocationTh2 cytokine profileXenograft model in SCID mice
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_2098 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Pleural effusion[UBERON:UBERON_0000175] |
Donor Information
| Age | 24 |
|---|---|
| Age Category | Adult |
| Sex | Female |
Disease Information
| Disease | ALK-positive anaplastic large cell lymphoma |
|---|---|
| Lineage | Lymphoid |
| Subtype | Anaplastic Large-Cell Lymphoma ALK Positive |
| OncoTree Code | ALCLALKP |
DepMap Information
| Source Type | Academic lab |
|---|---|
| Source ID | ACH-001111_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| Gene fusion | ALK | NPM1-ALK | - | - | PubMed=9121481, PubMed=7824924 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
10,12
D13S317
8,12
D16S539
10,11
D18S51
15,16
D19S433
12,14
D21S11
31.2,33.2
D2S1338
22,25
D3S1358
17,18
D5S818
12
D7S820
10,11
D8S1179
10,14
FGA
19,27
Penta D
10,11
Penta E
12
TH01
9.3
TPOX
8,11
vWA
16,17
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Characterization and diagnostic application of genomic NPM-ALK fusion sequences in anaplastic large-cell lymphoma.
Zimmermann M., Singh V.K., Metzler M., Damm-Welk C.
Oncotarget 9:26543-26555(2018).
Malignant hematopoietic cell lines: in vitro models for the study of anaplastic large-cell lymphoma.
Drexler H.G., MacLeod R.A.F.
Leukemia 18:1569-1571(2004).