LSHomo sapiens (Human)Cancer cell line

The LS cell line is a human neuroblastoma cell line established from an abdominal tumor of a 16-month-old girl. It exhibits a hyperdiploid karyotype with trisomy 17, a derivative chromosome 5, and two large marker-chromosomes with homogeneously stained regions (HSRs). These HSRs contain amplified N-myc gene copies, as demonstrated by in situ hybridization and Southern blot analysis. The cell line shows a 30-fold amplification of N-myc compared to other cell lines, with enhanced expression levels. The karyotype remains stable over 50 passages in vitro, indicating genetic stability. The LS cell line is valuable for studying gene amplification mechanisms, N-myc oncogene function, and neuroblastoma progression.