NMBHomo sapiens (Human)Cancer cell line

🤖 AI SummaryBased on 12 publications

Quick Overview

Human neuroblastoma cell line with specific opiate binding sites.

Detailed Summary

The NMB cell line is a human neuroblastoma cell line established from a 2-year-old child with metastatic disease. It exhibits specific opiate binding sites, characterized by high stereospecificity for delta-opioid peptides such as D-Ala2-D-Leu5 enkephalin and D-Thr2-D-Leu5 enkephalin. These binding sites are sensitive to trypsin and heat, and show a sodium-dependent decrease in agonist binding. The cell line has been used to study opioid receptor interactions and has been karyotyped to confirm its human origin and genetic stability. Research on NMB has contributed to understanding the pharmacology of opioid peptides and their receptors in neuroblastoma.

Research Applications

Opioid receptor studiesNeuroblastoma cell lineOpiate binding site characterization

Key Characteristics

Specific opiate binding sitesDelta-opioid receptor activitySensitivity to trypsin and heatSodium-dependent binding modulation

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2143
Species	Homo sapiens (Human)
Tissue Source	Bone marrow[UBERON:UBERON_0002371]

Donor Information

Age Category	Pediatric
Sex	Female
Subtype Features	MYC_Amplified

Disease Information

Disease	Neuroblastoma
Lineage	Peripheral Nervous System
Subtype	Neuroblastoma
OncoTree Code	NBL

DepMap Information

Source Type	DSMZ
Source ID	ACH-001367_source

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,12

D13S317

11,12

D16S539

9,13

D18S51

D19S433

11,13

D21S11

28,29

D2S1338

20,23

D3S1358

15,17

D5S818

D7S820

D8S1179

FGA

22,25

Penta D

5,11

Penta E

13,16

TH01

7,8

TPOX

7,9

vWA

17,18,20

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.

Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.

Sci. Data 4:170033-170033(2017).

DOI PubMed PMC

Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.

Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.

Transl. Oncol. 6:685-696(2013).

DOI PubMed PMC

PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.

Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.

Int. J. Cancer 131:1556-1568(2012).

DOI PubMed PMC

NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.

Messiaen L.M., Versteeg R., Bernards R.

Cell 142:218-229(2010).

DOI PubMed PMC

Identification of ALK as a major familial neuroblastoma predisposition gene.

Maris J.M.

Nature 455:930-935(2008).

DOI PubMed PMC

Mutations in PIK3CA are infrequent in neuroblastoma.";

Dam V., Morgan B.T., Mazanek P., Hogarty M.D.

BMC Cancer 6:177.1-177.10(2006).

DOI PubMed PMC

High-resolution detection and mapping of genomic DNA alterations in neuroblastoma.

Maris J.M.

Genes Chromosomes Cancer 43:390-403(2005).

DOI PubMed

Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.

Salwen H.R., Laureys G., Manoel N., De Paepe A., Speleman F.

Genes Chromosomes Cancer 32:126-135(2001).

DOI PubMed

Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour.

Gilbert F., Brodeur G.M., Goldstein M.N., Trent J.M.

Nature 305:245-248(1983).

DOI PubMed

NMB: a human neuroblastoma cell line with specific opiate binding sites.

Ard M.D., Goldstein M.N., Nash D.R., Gottlieb D.I.

Biochem. Biophys. Res. Commun. 133:60-66(1985).

DOI PubMed

Chromosomal aberrations in human neuroblastomas.";

Brodeur G.M., Sekhon G.S., Goldstein M.N.

Cancer 40:2256-2263(1977).

DOI PubMed

Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line.

Balaban-Malenbaum G.B., Gilbert F.

Science 198:739-741(1977).

DOI PubMed

Tumor cell lines of the peripheral nervous system.";

Israel M.A., Thiele C.J.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.43-78; Academic Press; New York; USA (1994).

DOI

Homogeneously staining regions and double minute chromosomes, prevalent cytogenetic abnormalities of human neuroblastoma cells.

Biedler J.L., Meyers M.B., Spengler B.A.

(In book chapter) Advances in cellular neurobiology, Vol. 4; Fedoroff S., Hertz L. (eds.); pp.267-307; Academic Press; New York; USA (1983).

DOI