PLB-985Homo sapiens (Human)Cancer cell line

Also known as: PLB985, PLB 985

🤖 AI SummaryBased on 7 publications

Quick Overview

Human myeloid leukemia cell line with applications in CGD research.

Detailed Summary

PLB-985 is a human myeloid leukemia cell line derived from a female patient with relapsed myeloid leukemia. It is widely used in research on Chronic Granulomatous Disease (CGD), particularly in studying the gp91phox and p47phox genes. The cell line is capable of differentiating into granulocytic and monocytic lineages, making it a valuable model for investigating the molecular mechanisms of CGD. PLB-985 has been utilized in gene targeting studies to disrupt the X-linked gp91phox gene, enabling the development of in vitro models for CGD. Additionally, it has been employed in CRISPR/Cas9-mediated gene therapy vector development for p47phox-deficient CGD. The cell line's ability to generate reactive oxygen species (ROS) upon differentiation makes it a key tool for studying respiratory burst activity in phagocytes.

Research Applications

Chronic Granulomatous Disease (CGD) researchGene targeting studiesCRISPR/Cas9-mediated gene therapy developmentRespiratory burst activity analysis

Key Characteristics

Capable of granulocytic and monocytic differentiationUsed in studying gp91phox and p47phox gene mutationsModel for X-linked and autosomal recessive CGDSupports in vitro gene therapy vector testing

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2162
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	35
Age Category	Adult
Sex	Female
Race	caucasian

Disease Information

Disease	Acute myeloid leukemia
Lineage	Myeloid
Subtype	Acute Myeloid Leukemia
OncoTree Code	AML

DepMap Information

Source Type	Academic lab
Source ID	ACH-000034_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	NRAS	p.Gln61Leu (c.182A>T)	Unspecified	-	PubMed=26214590
MutationSimple	CDKN2A	p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T)	Homozygous	-	from parent cell line HL-60
Gene deletion	TP53	-	Homozygous	2 out of 3 copies	from parent cell line HL-60

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

13,14

D13S317

8,11

D16S539

D18S51

14,15

D19S433

D21S11

29,30

D2S1338

D3S1358

D5S818

D7S820

11,12

D8S1179

FGA

22,24

Penta D

10,11

Penta E

13,14

TH01

7,8

TPOX

8,11

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Anti-leukemic properties of histamine in monocytic leukemia: the role of NOX2.

Nilsson J.A., Stahlberg A., Thoren F.B., Hellstrand K., Martner A.

Front. Oncol. 8:218.1-218.12(2018).

DOI PubMed PMC

CRISPR/Cas9-generated p47(phox)-deficient cell line for chronic granulomatous disease gene therapy vector development.

Wrona D., Siler U., Reichenbach J.

Sci. Rep. 7:44187-44187(2017).

DOI PubMed PMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOI PubMed PMC

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

DOI PubMed PMC

Check your cultures! A list of cross-contaminated or misidentified cell lines.

Freshney R.I.

Int. J. Cancer 127:1-8(2010).

DOI PubMed

False leukemia-lymphoma cell lines: an update on over 500 cell lines.

Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.

Leukemia 17:416-426(2003).

DOI PubMed

Gene targeting of X chromosome-linked chronic granulomatous disease locus in a human myeloid leukemia cell line and rescue by expression of recombinant gp91phox.

Zhen L., King A.A.J., Xiao Y.-H., Chanock S.J., Orkin S.H., Dinauer M.C.

Proc. Natl. Acad. Sci. U.S.A. 90:9832-9836(1993).

DOI PubMed PMC

Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity.

Tucker K.A., Lilly M.B., Heck L. Jr., Rado T.A.

Blood 70:372-378(1987).

DOI PubMed

Web Resources

thermofisher.com iclac.org