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RD-ESHomo sapiens (Human)Cancer cell line

Also known as: RDES-1, RDES

🤖 AI SummaryBased on 15 publications

Quick Overview

RD-ES is a human Ewing sarcoma cell line used in cancer research.

Detailed Summary

RD-ES is a human Ewing sarcoma cell line derived from a bone tumor. It is commonly used in research to study the molecular mechanisms of Ewing sarcoma, including the role of specific gene fusions and mutations. The cell line has been characterized for its genetic profile, including the presence of EWSR1-FLI-1 fusion and mutations in genes such as STAG2 and TP53. RD-ES is utilized in studies related to drug sensitivity, genomic alterations, and the development of targeted therapies for Ewing sarcoma. Research involving RD-ES has contributed to understanding the genetic and molecular basis of this aggressive cancer, aiding in the identification of potential therapeutic targets.

Research Applications

Molecular mechanisms of Ewing sarcomaGene fusion analysis (EWSR1-FLI-1)Mutation profiling (STAG2, TP53)Drug sensitivity testingGenomic alterations in cancerTargeted therapy development

Key Characteristics

EWSR1-FLI-1 fusionSTAG2 mutationsTP53 mutationsHigh genomic instabilityUsed in drug screening assays

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2169
Species	Homo sapiens (Human)
Tissue Source	Bone, humerus[UBERON:UBERON_0000976]

Donor Information

Age	19
Age Category	Adult
Sex	Male
Race	caucasian
Subtype Features	EWS-FLI

Disease Information

Disease	Ewing sarcoma
Lineage	Bone
Subtype	Ewing Sarcoma
OncoTree Code	ES

DepMap Information

Source Type	ATCC
Source ID	ACH-000041_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg273Cys (c.817C>T)	Homozygous	-	PubMed=35933914
Gene fusion	EWSR1	EWSR1-FLI1, EWS-FLI1	-	Type 1 fusion	PubMed=15150091
Gene deletion	CDKN2A	-	Homozygous	Possible	PubMed=26870271

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

11

D13S317

11,12

D16S539

9,11

D18S51

14,18

D19S433

13,14

D21S11

28

D2S1338

19,20

D3S1358

15

D5S818

11

D7S820

10

D8S1179

13

FGA

21,25

Penta D

9,12

Penta E

11,13

SE33

17,18

TH01

7

TPOX

9,11

vWA

17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

EZH2 inhibition in Ewing sarcoma upregulates GD2 expression for targeting with gene-modified T cells.

Muller I., Walles H., Hartmann W., Rossig C.

Mol. Ther. 27:933-946(2019).

T cell infiltration into Ewing sarcomas is associated with local expression of immune-inhibitory HLA-G.

Meltzer J., Farwick N., Greune L., Rossig C.

Oncotarget 9:6536-6549(2018).

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.

Schmidt T., Szuhai K., Hogendoorn P.C.W.

Eur. J. Cancer 51:2624-2633(2015).

Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.

Harris E., Monks A., Morris J.

Mol. Cancer Ther. 14:2452-2462(2015).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

Genomic landscape of Ewing sarcoma defines an aggressive subtype with co-association of STAG2 and TP53 mutations.

Zhang J.-H., Delattre O.

Cancer Discov. 4:1342-1353(2014).

The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.

Catchpoole D., Llombart-Bosch A., Waldman T., Khan J.

PLoS Genet. 10:E1004475-E1004475(2014).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways.

Borsu L., Barr F.G., Ladanyi M.

Clin. Cancer Res. 18:748-757(2012).

1q gain and CDT2 overexpression underlie an aggressive and highly proliferative form of Ewing sarcoma.

Debiec-Rychter M., Schaefer K.-L., de Alava E.

Oncogene 31:1287-1298(2012).

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Haber D.A.

Cancer Res. 70:2158-2164(2010).

Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.

Buerger H., Aigner T., Gabbert H.E., Poremba C.

Genes Chromosomes Cancer 49:40-51(2010).

Microdeletions in 9p21.3 induce false negative results in CDKN2A FISH analysis of Ewing sarcoma.

Savola S., Nardi F., Scotlandi K., Picci P., Knuutila S.

Cytogenet. Genome Res. 119:21-26(2007).

Identification of side population cells (stem-like cell population) in pediatric solid tumor cell lines.

Hayashi Y.

J. Pediatr. Surg. 42:2040-2045(2007).

Analysis of the expression of cell cycle regulators in Ewing cell lines: EWS-FLI-1 modulates p57KIP2and c-Myc expression.

Weissman B.E., Delattre O.

Oncogene 20:3258-3265(2001).

Molecular analysis of Ewing's sarcoma: another fusion gene, EWS-E1AF, available for diagnosis.

Hata J.-i.

Jpn. J. Cancer Res. 89:703-711(1998).

Narrow spectrum of infrequent p53 mutations and absence of MDM2 amplification in Ewing tumours.

Salzer-Kuntschik M., Gadner H.

Oncogene 8:2683-2690(1993).

Mutations of the p53 gene are involved in Ewing's sarcomas but not in neuroblastomas.

Kamoshita S., Hanada R., Yamamoto K., Hongo T., Yamada M., Tsuchida Y.

Cancer Res. 53:5284-5288(1993).

EWS-erg and EWS-Fli1 fusion transcripts in Ewing's sarcoma and primitive neuroectodermal tumors with variant translocations.

Nycum L.M., Emanuel B.S., Evans G.A.

J. Clin. Invest. 94:489-496(1994).

Systematic multi-omics profiling of Ewing sarcoma cell lines.";

Orth M.F.

Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany.

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