SET-2Homo sapiens (Human)Cancer cell line

Also known as: SET2, Set2, Set-2

🤖 AI SummaryBased on 8 publications

Quick Overview

SET-2 is a human megakaryoblastic cell line derived from a patient with leukemic transformation of essential thrombocythemia, k...

Detailed Summary

SET-2 is a human megakaryoblastic cell line established from the peripheral blood of a patient with leukemic transformation of essential thrombocythemia (ET). This cell line is notable for its spontaneous maturation into multinucleated megakaryocytes and the production of platelet-like particles in culture. SET-2 expresses the JAK2 V617F mutation, which is associated with myeloproliferative disorders. It is also characterized by the expression of various surface markers, including CD4, CD7, CD13, CD33, CD34, CD36, CD38, CD41, CD61, and c-mpl. The cell line has been used in studies related to megakaryocyte differentiation, platelet production, and the role of the JAK2 V617F mutation in disease progression. Additionally, SET-2 has been utilized in research on the molecular mechanisms of myeloproliferative neoplasms and the development of targeted therapies.

Research Applications

Study of megakaryocyte differentiationInvestigation of platelet-like particle productionAnalysis of JAK2 V617F mutation in myeloproliferative disordersDevelopment of targeted therapies for myeloproliferative neoplasms

Key Characteristics

Spontaneous maturation into multinucleated megakaryocytesProduction of platelet-like particlesExpression of JAK2 V617F mutationExpression of surface markers including CD4, CD7, CD13, CD33, CD34, CD36, CD38, CD41, CD61, and c-mpl

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2187
Species	Homo sapiens (Human)
Tissue Source	Peripheral blood[UBERON:UBERON_0000178]

Donor Information

Age	71
Age Category	Adult
Sex	Female

Disease Information

Disease	Acute megakaryoblastic leukemia
Lineage	Myeloid
Subtype	Acute Megakaryoblastic Leukemia
OncoTree Code	AMKL

DepMap Information

Source Type	DSMZ
Source ID	ACH-000195_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg248Trp (c.742C>T)	Homozygous	Somatic mutation acquired during proliferation	from parent cell line VCaP
MutationSimple	TP53	c.559+2T>A	Heterozygous	Splice donor mutation	Unknown, Unknown
MutationSimple	JAK2	p.Val617Phe (c.1849G>T)	Homozygous	-	from parent cell line UKE-1
MutationSimple	DNMT3A	p.Arg882His (c.2645G>A)	Heterozygous	-	PubMed=30304769

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

D13S317

8,10

D16S539

9,10

D18S51

13,16

D19S433

D21S11

D2S1338

18,23

D3S1358

14,16

D5S818

D7S820

10,12

D8S1179

13,14

FGA

19,23

Penta D

10,11

Penta E

16,20

TH01

7,9.3

TPOX

vWA

14,17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

DNMT3A R882H mutation in acute myeloid leukemia cell line SET-2.";

Uphoff C.C., Zaborski M., Drexler H.G.

Leuk. Res. 88:106270.1-106270.3(2020).

DOI PubMed

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Screening human cell lines for viral infections applying RNA-Seq data analysis.

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

PLoS ONE 14:E0210404-E0210404(2019).

DOI PubMed PMC

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

Yang H.H., Koeffler H.P.

BMC Cancer 18:940.1-940.13(2018).

DOI PubMed PMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOI PubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

Leukemia 20:471-476(2006).

DOI PubMed

Establishment and characterization of a new human megakaryoblastic cell line (SET-2) that spontaneously matures to megakaryocytes and produces platelet-like particles.

Shimotakahara S., Matsumura I., Hanada S., Arima T.

Leukemia 14:142-152(2000).

DOI PubMed