SK-GT-2Homo sapiens (Human)Cancer cell line
Also known as: SKGT2
🤖 AI SummaryBased on 2 publications
Quick Overview
Human gastric cancer cell line with p53 mutations
Detailed Summary
SK-GT-2 is a human gastric cancer cell line established from a primary adenocarcinoma of the distal esophagus. It exhibits mutations in the p53 gene, specifically a point mutation at codon 175 in exon 5, leading to an arginine to histidine amino acid substitution. This cell line shows resistance to chemotherapeutic agents such as 5-fluorouracil (5-FU), mitomycin C (MMC), and cisplatin (CDDP), likely due to the presence of mutant p53 protein. Studies have demonstrated that wild-type p53 protein levels increase in response to these drugs through post-translational stabilization, whereas mutant p53 levels remain relatively unchanged. The cell line is used in research focusing on the role of p53 mutations in chemosensitivity and cancer progression.
Research Applications
Cancer researchChemosensitivity studiesp53 mutation analysis
Key Characteristics
p53 mutation at codon 175Resistance to 5-FU, MMC, and CDDPPost-translational stabilization of wild-type p53
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_2194 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Stomach, fundus[UBERON:UBERON_0001160] |
Donor Information
| Age | 72 |
|---|---|
| Age Category | Adult |
| Sex | Male |
Disease Information
| Disease | Gastric fundus carcinoma |
|---|---|
| Lineage | Esophagus/Stomach |
| Subtype | Stomach Adenocarcinoma |
| OncoTree Code | STAD |
DepMap Information
| Source Type | Sigma-Aldrich |
|---|---|
| Source ID | ACH-001653_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Arg175His (c.524G>A) | Unspecified | Somatic mutation acquired during proliferation | from parent cell line YCC-3 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
10
D13S317
12,13
D16S539
11,12
D18S51
14,15
D19S433
12
D21S11
32
D2S1338
16,19
D3S1358
15
D5S818
10
D7S820
9,10
D8S1179
15
FGA
26
Penta D
9
Penta E
15,17
TH01
8,9
TPOX
9,12
vWA
15,18
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Pan-cancer proteomic map of 949 human cell lines.";
Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Cancer Cell 40:835-849.e8(2022).
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
Cancer Res. 79:1263-1273(2019).
A landscape of pharmacogenomic interactions in cancer.";
Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
Cell 166:740-754(2016).
Characterization of cell lines established from human gastric-esophageal adenocarcinomas. Biologic phenotype and invasion potential.
Albino A.P.
Cancer 72:649-657(1993).