SK-GT-4Homo sapiens (Human)Cancer cell line

Also known as: SK4, SKGT4, SJGT4

🤖 AI SummaryBased on 8 publications

Quick Overview

Human esophageal adenocarcinoma cell line derived from gastric fundus carcinoma.

Detailed Summary

SK-GT-4 is a human esophageal adenocarcinoma cell line established from a primary adenocarcinoma of the gastroesophageal junction. It is part of a panel of 10 verified esophageal adenocarcinoma cell lines that have been authenticated through genotyping and genetic analysis. These cell lines are widely used in cancer research for studying molecular mechanisms, drug development, and therapeutic strategies. SK-GT-4 has been utilized in studies examining the mutational status of p53 protein and its correlation with chemosensitivity to various chemotherapeutic agents. Additionally, it has been involved in research on the role of cancer stem cell pathways and the impact of targeted therapies on tumor growth and resistance. The cell line is known for its genetic stability and relevance to human esophageal adenocarcinoma, making it a valuable tool for in vitro studies.

Research Applications

Molecular mechanisms of carcinogenesisDrug development and chemosensitivity testingCancer stem cell pathwaysTargeted therapy research

Key Characteristics

Genetically stableRelevant to human esophageal adenocarcinomaUsed in p53 mutation and chemosensitivity studies

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2195
Species	Homo sapiens (Human)
Tissue Source	Esophagus, distal[UBERON:UBERON_0013473]

Donor Information

Age	89
Age Category	Adult
Sex	Male

Disease Information

Disease	Adenocarcinoma of the esophagus
Lineage	Esophagus/Stomach
Subtype	Esophageal Adenocarcinoma
OncoTree Code	ESCA

DepMap Information

Source Type	Sigma-Aldrich
Source ID	ACH-001654_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Gln100Ter (c.298C>T)	Homozygous	-	from parent cell line SK-GT-4
MutationSimple	PIK3CA	p.Glu542Lys (c.1624G>A)	Unspecified	-	PubMed=31541927
MutationSimple	KRAS	p.Gly12Ala (c.35G>C)	Unspecified	-	PubMed=21173094

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,15

D13S317

9,10

D16S539

11,12,13

D18S51

D19S433

13,14

D21S11

31.2

D2S1338

16,19

D3S1358

D5S818

D7S820

7,11

D8S1179

FGA

22,23

Penta D

Penta E

TH01

6,9.3

TPOX

8,10

vWA

17,19

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOI PubMed PMC

Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.

Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.

Nature 568:511-516(2019).

DOI PubMed

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Whole-genome sequencing of nine esophageal adenocarcinoma cell lines.";

Lynch A.G., Edwards P.A.W., Fitzgerald R.C.

F1000Research 5:1336.1-1336.12(2016).

DOI PubMed PMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOI PubMed PMC

ABT-263 induces apoptosis and synergizes with chemotherapy by targeting stemness pathways in esophageal cancer.

Jin J.-K., Ma L., Zhu H.-T., Skinner H.D., Johnson R.L., Ajani J.A.

Oncotarget 6:25883-25896(2015).

DOI PubMed PMC

Translational research on esophageal adenocarcinoma: from cell line to clinic.

Boonstra J.J., Tilanus H.W., Dinjens W.N.M.

Dis. Esophagus 28:90-96(2015).

DOI PubMed

An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.

Kelleher D.P.

Carcinogenesis 31:936-945(2010).

DOI PubMed

Verification and unmasking of widely used human esophageal adenocarcinoma cell lines.

Shimada Y., van Dekken H., Tilanus H.W., Dinjens W.N.M.

J. Natl. Cancer Inst. 102:271-274(2010).

DOI PubMed PMC

Gene expression profiling in human esophageal cancers using cDNA microarray.

Itami A., Yamasaki S., Imamura M.

Biochem. Biophys. Res. Commun. 286:792-801(2001).

DOI PubMed

Characterization of cell lines established from human gastric-esophageal adenocarcinomas. Biologic phenotype and invasion potential.

Albino A.P.

Cancer 72:649-657(1993).

DOI PubMed

The mutational status of p53 protein in gastric and esophageal adenocarcinoma cell lines predicts sensitivity to chemotherapeutic agents.

Schwartz G.K., Blundell M.L., Altorki N.K., Kelsen D.P., Albino A.P.

Int. J. Cancer 64:37-46(1995).

DOI PubMed