SKNO-1Homo sapiens (Human)Cancer cell line

Also known as: SKNO1, Skno-1

🤖 AI SummaryBased on 9 publications

Quick Overview

SKNO-1 is a human myeloid leukemia cell line derived from a patient with t(8;21) and monosomy 17, used in leukemia research.

Detailed Summary

SKNO-1 is a human myeloid leukemia cell line established from the bone marrow of a 22-year-old male with acute myeloblastic leukemia (AML) M2. It carries the t(8;21) translocation and monosomy 17, which are associated with disease progression and resistance to therapy. The cell line is dependent on granulocyte-macrophage colony-stimulating factor (GM-CSF) for growth and exhibits overexpression of the p53 protein, which is linked to poor prognosis in leukemia. SKNO-1 is used in studies of leukemogenesis, molecular mechanisms of myeloid malignancies, and drug development for AML. It is also utilized in research on the role of p53 mutations in disease progression and in identifying therapeutic targets for leukemia treatment.

Research Applications

Leukemogenesis studiesMolecular mechanisms of myeloid malignanciesDrug development for AMLp53 mutation analysis in leukemia progression

Key Characteristics

t(8;21) translocationMonosomy 17GM-CSF-dependent growthOverexpression of p53 protein

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2196
Species	Homo sapiens (Human)
Tissue Source	Bone marrow[UBERON:UBERON_0002371]

Donor Information

Age	22
Age Category	Adult
Sex	Male

Disease Information

Disease	Acute myeloblastic leukemia with maturation
Lineage	Myeloid
Subtype	AML with Maturation
OncoTree Code	AM

DepMap Information

Source Type	DSMZ
Source ID	ACH-001656_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.His368Arg (c.1103G>A)	Homozygous	-	Unknown, PubMed=7772516
MutationSimple	TP53	p.Arg248Gln (c.743G>A)	Unspecified	Somatic mutation acquired during proliferation	PubMed=20575032
MutationSimple	KIT	p.Asn822Lys (c.2466T>A) (N818K)	Unspecified	-	Wistar
Gene fusion	RUNX1	RUNX1-RUNX1T1, AML1-ETO	-	-	PubMed=15843827, PubMed=11753612

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

12,13

D13S317

8,11

D16S539

10,11

D18S51

13,14

D19S433

13,15

D21S11

29,30

D2S1338

D3S1358

D5S818

10,13

D7S820

10,12

D8S1179

14,15

FGA

Penta D

Penta E

11,12

TH01

TPOX

8,9

vWA

14,17

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

The LL-100 panel: 100 cell lines for blood cancer studies.";

MacLeod R.A.F., Nagel S., Steube K.G., Uphoff C.C., Drexler H.G.

Sci. Rep. 9:8218-8218(2019).

DOI PubMed PMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOI PubMed PMC

Two cell lines of t(8;21) acute myeloid leukemia with activating KIT exon 17 mutation: models for the 'second hit' hypothesis.

Schwabe M., Lubbert M.

Leukemia 22:1792-1794(2008).

DOI PubMed

Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.

Fioretos T.

Leukemia 19:1042-1050(2005).

DOI PubMed

Molecular characterization of genomic AML1-ETO fusions in childhood leukemia.

Dicks B.M., Wiencke J.K., Wiemels J.L.

Leukemia 15:1906-1913(2001).

DOI PubMed

p53 alterations in human leukemia-lymphoma cell lines: in vitro artifact or prerequisite for cell immortalization?

Uphoff C.C.

Leukemia 14:198-206(2000).

DOI PubMed

Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.

Aizawa Y., Ueda R., Seto M.

Jpn. J. Cancer Res. 89:712-718(1998).

DOI PubMed PMC

Establishment of a myeloid leukaemic cell line (SKNO-1) from a patient with t(8;21) who acquired monosomy 17 during disease progression.

Murayama T., Koizumi T., Nishimura R., Isobe T., Chihara K.

Br. J. Haematol. 89:805-811(1995).

DOI PubMed

The leukemia-lymphoma cell line factsbook.";

Drexler H.G.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001).

DOI