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TC-71Homo sapiens (Human)Cancer cell line

Also known as: GM11654, TC71

🤖 AI SummaryBased on 13 publications

Quick Overview

TC-71 is a human Ewing sarcoma cell line used in cancer research.

Detailed Summary

TC-71 is a human Ewing sarcoma cell line derived from a bone tumor. It is widely used in research to study the genetic and molecular mechanisms of Ewing sarcoma, particularly focusing on the EWSR1-FLI1 fusion gene. This cell line has been utilized in studies examining the role of STAG2 mutations and their association with tumor progression and prognosis. TC-71 is also employed in drug sensitivity assays to evaluate potential therapeutic agents for Ewing sarcoma treatment. The cell line's genomic profile, including mutations in TP53 and CDKN2A, has been characterized, contributing to the understanding of its biological behavior and response to various treatments.

Research Applications

Genomic analysis of Ewing sarcomaDrug sensitivity testingStudy of EWSR1-FLI1 fusion geneInvestigation of STAG2 mutationsAnalysis of TP53 and CDKN2A mutations

Key Characteristics

EWSR1-FLI1 fusion geneSTAG2 mutationsTP53 mutationsCDKN2A deletions
Generated on 6/18/2025

Basic Information

Database IDCVCL_2213
SpeciesHomo sapiens (Human)
Tissue SourceBone, humerus[UBERON:UBERON_0000976]

Donor Information

Age22
Age CategoryAdult
SexMale

Disease Information

DiseaseEwing sarcoma
LineageBone
SubtypeEwing Sarcoma
OncoTree CodeES

DepMap Information

Source TypeDSMZ
Source IDACH-000424_source

Known Sequence Variations

TypeGene/ProteinDescriptionZygosityNoteSource
MutationSimpleTP53p.Gly245Cys (c.733G>T)HeterozygousSomatic mutation acquired during proliferationPubMed=18487078, PubMed=16020667, PubMed=15287027, PubMed=12884349, PubMed=1394225
MutationSimpleTP53p.Arg213Ter (c.637C>T)Unspecified-Unknown
Gene fusionEWSR1EWSR1-FLI1, EWS-FLI1-Type 1 fusionPubMed=15150091
Gene deletionCDKN2A-HomozygousPossiblePubMed=26870271

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin
X
CSF1PO
10
D13S317
11,12
D16S539
11,14
D18S51
12,15
D19S433
14
D21S11
29,30
D2S1338
20,24
D3S1358
15,17
D5S818
10
D7S820
10
D8S1179
10
FGA
24,26
Penta D
11,14
Penta E
12
SE33
21,30.2
TH01
9.3
TPOX
8,9
vWA
17
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines

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Publications

Pan-cancer proteomic map of 949 human cell lines.";

Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.

Cancer Cell 40:835-849.e8(2022).

DOIPubMedPMC

Quantitative proteomics of the Cancer Cell Line Encyclopedia.";

Sellers W.R., Gygi S.P.

Cell 180:387-402.e16(2020).

DOIPubMedPMC

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOIPubMedPMC

Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.

Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.

Nature 568:511-516(2019).

DOIPubMed

FET family fusion oncoproteins target the SWI/SNF chromatin remodeling complex.

Fagman H., Stahlberg A., Aman P.

EMBO Rep. 20:e45766.1-e45766.15(2019).

DOIPubMedPMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOIPubMedPMC

EZH2 inhibition in Ewing sarcoma upregulates GD2 expression for targeting with gene-modified T cells.

Muller I., Walles H., Hartmann W., Rossig C.

Mol. Ther. 27:933-946(2019).

DOIPubMedPMC

T cell infiltration into Ewing sarcomas is associated with local expression of immune-inhibitory HLA-G.

Meltzer J., Farwick N., Greune L., Rossig C.

Oncotarget 9:6536-6549(2018).

DOIPubMedPMC

Characterization of human cancer cell lines by reverse-phase protein arrays.

Liang H.

Cancer Cell 31:225-239(2017).

DOIPubMedPMC

A landscape of pharmacogenomic interactions in cancer.";

Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

Cell 166:740-754(2016).

DOIPubMedPMC

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

DOIPubMedPMC

CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.

Schmidt T., Szuhai K., Hogendoorn P.C.W.

Eur. J. Cancer 51:2624-2633(2015).

DOIPubMed

Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.

Harris E., Monks A., Morris J.

Mol. Cancer Ther. 14:2452-2462(2015).

DOIPubMedPMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOIPubMedPMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOIPubMed

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

DOIPubMed

Genomic landscape of Ewing sarcoma defines an aggressive subtype with co-association of STAG2 and TP53 mutations.

Zhang J.-H., Delattre O.

Cancer Discov. 4:1342-1353(2014).

DOIPubMedPMC

The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.

Catchpoole D., Llombart-Bosch A., Waldman T., Khan J.

PLoS Genet. 10:E1004475-E1004475(2014).

DOIPubMedPMC

Characterization and drug resistance patterns of Ewing's sarcoma family tumor cell lines.

Jenabi J., Ji L.-Y., Triche T.J., Lawlor E.R., Reynolds C.P.

PLoS ONE 8:E80060-E80060(2013).

DOIPubMedPMC

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOIPubMedPMC

Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways.

Borsu L., Barr F.G., Ladanyi M.

Clin. Cancer Res. 18:748-757(2012).

DOIPubMedPMC

1q gain and CDT2 overexpression underlie an aggressive and highly proliferative form of Ewing sarcoma.

Debiec-Rychter M., Schaefer K.-L., de Alava E.

Oncogene 31:1287-1298(2012).

DOIPubMed

National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.

Reynolds C.P.

Pediatr. Blood Cancer 56:239-249(2011).

DOIPubMedPMC

CD99 inhibits neural differentiation of human Ewing sarcoma cells and thereby contributes to oncogenesis.

Lessnick S.L., Picci P., Scotlandi K.

J. Clin. Invest. 120:668-680(2010).

DOIPubMedPMC

Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.

Buerger H., Aigner T., Gabbert H.E., Poremba C.

Genes Chromosomes Cancer 49:40-51(2010).

DOIPubMed

Microdeletions in 9p21.3 induce false negative results in CDKN2A FISH analysis of Ewing sarcoma.

Savola S., Nardi F., Scotlandi K., Picci P., Knuutila S.

Cytogenet. Genome Res. 119:21-26(2007).

DOIPubMed

Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.

Gabbert H.E., Poremba C.

Cancer Res. 64:3395-3405(2004).

DOIPubMed

The expression of ccn3(nov) gene in musculoskeletal tumors.";

Picci P., Scotlandi K.

Am. J. Pathol. 160:849-859(2002).

DOIPubMedPMC

Gene expression profiling of alveolar rhabdomyosarcoma with cDNA microarrays.

Smith P.D., Jiang Y., Gooden G.C., Trent J.M., Meltzer P.S.

Cancer Res. 58:5009-5013(1998).

PubMed

Immunostaining of the p30/32MIC2 antigen and molecular detection of EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor.

Lollini P.-L., Picci P., Bertoni F., Baldini N.

Hum. Pathol. 27:408-416(1996).

DOIPubMed

EWS-erg and EWS-Fli1 fusion transcripts in Ewing's sarcoma and primitive neuroectodermal tumors with variant translocations.

Nycum L.M., Emanuel B.S., Evans G.A.

J. Clin. Invest. 94:489-496(1994).

DOIPubMedPMC

Indistinguishable patterns of protooncogene expression in two distinct but closely related tumors: Ewing's sarcoma and neuroepithelioma.

Israel M.A.

Cancer Res. 48:4307-4311(1988).

PubMed

Cytogenetic characterization of selected small round cell tumors of childhood.

Tsai S., Israel M.A.

Cancer Genet. Cytogenet. 21:185-208(1986).

DOIPubMed

Systematic multi-omics profiling of Ewing sarcoma cell lines.";

Orth M.F.

Thesis PhD (2021); Ludwig Maximilians University of Munich; Munich; Germany.

DOI

Web Resources

cccells.orgcccells.orgpptpinvitro.orgtcpaportal.org