Back to Cell Types

143BHomo sapiens (Human)Cancer cell line

Also known as: GM05887A, GM05887, HOS-143b, HOS-143B, 143TK-, 143BTK-, 143B.TK-, 143B TK-, 143 B, 143b, I43B

🤖 AI SummaryBased on 9 publications

Quick Overview

Osteosarcoma cell line with metastatic potential and genetic diversity.

Detailed Summary

The 143B cell line is a human osteosarcoma cell line derived from a bone tumor. It is known for its metastatic potential, particularly to the lungs, and has been extensively studied in research related to cancer metastasis and genetic diversity. This cell line is part of the EuroBoNet consortium and has been used in studies involving genomic and epigenetic alterations, as well as in drug sensitivity profiling. The 143B cell line is also notable for its role in understanding the mechanisms of metastasis and has been utilized in various studies to investigate the impact of genetic and epigenetic changes on tumor behavior. Additionally, it has been used in research on the immune response and the development of targeted therapies.

Research Applications

Metastasis researchGenomic and epigenetic alterationsDrug sensitivity profilingImmune response studiesTargeted therapy development

Key Characteristics

Metastatic potentialGenetic diversityUsed in EuroBoNet consortiumStudied for metastasis mechanismsUtilized in drug sensitivity studies

Generated on 6/18/2025

Basic Information

Database ID	CVCL_2270
Species	Homo sapiens (Human)
Tissue Source	Bone, right femur[UBERON:UBERON_0000981]

Donor Information

Age	13
Age Category	Pediatric
Sex	Female
Race	caucasian

Disease Information

Disease	Osteosarcoma
Lineage	Bone
Subtype	Osteosarcoma
OncoTree Code	OS

DepMap Information

Source Type	ATCC
Source ID	ACH-001001_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg156Pro (c.467G>C)	Unspecified	-	PubMed=32784519
MutationSimple	KRAS	p.Ala59Thr (c.175G>A)	Heterozygous	-	Unknown, Unknown, PubMed=10362137
MutationSimple	KRAS	p.Gly12Ser (c.34G>A)	Unspecified	-	PubMed=27004849
Gene deletion	CDKN2A	-	Homozygous	Possible	PubMed=26870271

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X

CSF1PO

12

D13S317

12

D16S539

10,13

D18S51

17

D19S433

13

D21S11

31.2,32.2

D2S1338

24,25

D3S1358

15

D5S818

13

D7S820

11,12

D8S1179

11,14

FGA

24

Penta D

9,10

Penta E

7,12

SE33

21

TH01

6

TPOX

11

vWA

18

Gene Expression Profile

Gene expression levels and statistical distribution

Loading cohorts...

Full DepMap dataset with combined data across cell lines

Loading gene expression data...

Publications

Establishment and characterization of single and triple-agent resistant osteosarcoma cell lines.

Low K., Hills F., Roberts H.C., Stordal B.

Adv. Biol. (Weinh.) 7:e2200194.1-e2200194.13(2023).

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

Positively selected enhancer elements endow osteosarcoma cells with metastatic competence.

Versteeg H.H., Stamatoyannopoulos J.A., Khanna C., Scacheri P.C.

Nat. Med. 24:176-185(2018).

An impaired respiratory electron chain triggers down-regulation of the energy metabolism and de-ubiquitination of solute carrier amino acid transporters.

Aretz I., Hardt C., Wittig I., Meierhofer D.

Mol. Cell. Proteomics 15:1526-1538(2016).

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Loewer M., Sahin U., Castle J.C.

Genome Med. 7:118.1-118.7(2015).

Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.

Luu H.H., Meltzer P.S., Khanna C.

Oncotarget 6:29469-29481(2015).

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

A comprehensive transcriptional portrait of human cancer cell lines.

Settleman J., Seshagiri S., Zhang Z.-M.

Nat. Biotechnol. 33:306-312(2015).

Integrative analysis reveals relationships of genetic and epigenetic alterations in osteosarcoma.

Myklebost O., Meza-Zepeda L.A.

PLoS ONE 7:E48262-E48262(2012).

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

Functional characterization of osteosarcoma cell lines provides representative models to study the human disease.

Hogendoorn P.C.W., Llombart-Bosch A., Cleton-Jansen A.-M.

Lab. Invest. 91:1195-1205(2011).

Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.

Buerger H., Aigner T., Gabbert H.E., Poremba C.

Genes Chromosomes Cancer 49:40-51(2010).

p53 functions as a cell cycle control protein in osteosarcomas.";

Bressac B., Ozturk M., Baker S.J., Vogelstein B., Friend S.H.

Mol. Cell. Biol. 10:5772-5781(1990).

Web Resources

thermofisher.com