H314Homo sapiens (Human)Cancer cell line
🤖 AI SummaryBased on 3 publications
Quick Overview
Human oral squamous cell carcinoma cell line with p53 mutations and HPV-16 presence.
Detailed Summary
The H314 cell line is derived from human oral squamous cell carcinoma and exhibits key characteristics relevant to cancer research. It expresses high levels of mutant p53 protein, with two distinct point mutations identified in the coding region of the p53 gene. These mutations are located at codon 176 (exon 5) and codon 373 (exon 11), suggesting potential roles in tumorigenesis. Additionally, H314 was found to contain low copy numbers of HPV-16 sequences, indicating a possible viral contribution to its malignant phenotype. The cell line is immortal, 3T3-independent, and shows anchorage-independent growth, making it a valuable model for studying oral cancer progression and viral interactions in carcinogenesis. H314 also demonstrates variable MHC antigen expression, with constitutive MHC class I but not class II antigens, and altered responses to IFN-γ stimulation. These features make H314 a useful tool for investigating the molecular mechanisms underlying oral squamous cell carcinoma.
Research Applications
p53 mutation analysisHPV interaction studiesoral cancer progressionMHC antigen expressionimmortal cell line model
Key Characteristics
immortal3T3-independentanchorage-independenthigh mutant p53 expressionHPV-16 presencevariable MHC class I/II expression
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_2460 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Oral cavity, floor of mouth[UBERON:UBERON_0003679] |
Donor Information
| Age | 82 |
|---|---|
| Age Category | Adult |
| Sex | Male |
Disease Information
| Disease | Squamous cell carcinoma of the oral cavity |
|---|---|
| Lineage | Head and Neck |
| Subtype | Oral Cavity Squamous Cell Carcinoma |
| OncoTree Code | OCSC |
DepMap Information
| Source Type | Sigma-Aldrich |
|---|---|
| Source ID | ACH-001508_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Lys373Glu (c.1117A>G) | Homozygous | - | PubMed=7633286 |
| MutationSimple | TP53 | p.Cys176Phe (c.527G>T) | Unspecified | - | PubMed=8221663 |
Gene Expression Profile
Gene expression levels and statistical distribution
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Publications
Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
Yeudall W.A., Paterson I.C., Patel V., Prime S.S.
Eur. J. Cancer B. Oral Oncol. 31:136-143(1995).
MHC antigen expression in human oral squamous carcinoma cell lines.";
Mutlu S., Matthews J.B., Midda M., Scully C., Prime S.S.
J. Pathol. 165:129-136(1991).