H357Homo sapiens (Human)Cancer cell line
🤖 AI SummaryBased on 4 publications
Quick Overview
Human oral squamous cell carcinoma cell line with p53 mutations and stem cell properties.
Detailed Summary
The H357 cell line is a human oral squamous cell carcinoma cell line derived from untreated intraoral squamous cell carcinomas. It exhibits frequent p53 mutations, which are associated with tobacco-related carcinogenesis. The cell line shows characteristics of stem cells, including the ability to form colonies with different morphologies, express stem cell markers like CD44, and form tumor spheres in suspension. H357 cells also demonstrate resistance to apoptosis, which may be linked to an extended G2 cell cycle phase. These properties make H357 a valuable model for studying the molecular mechanisms of oral cancer progression and therapeutic resistance.
Research Applications
Analysis of p53 mutations in oral cancerStudy of stem cell properties in carcinoma cellsInvestigation of apoptosis resistance mechanismsEvaluation of tumor sphere formation in suspension culture
Key Characteristics
Frequent p53 mutationsExpression of CD44 stem cell markerResistance to apoptosisAbility to form tumor spheresExtended G2 phase in cell cycle
Generated on 6/18/2025
Basic Information
| Database ID | CVCL_2462 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Oral cavity, tongue[UBERON:UBERON_0001723] |
Donor Information
| Age | 74 |
|---|---|
| Age Category | Adult |
| Sex | Male |
Disease Information
| Disease | Squamous cell carcinoma of the oral tongue |
|---|---|
| Lineage | Head and Neck |
| Subtype | Oral Cavity Squamous Cell Carcinoma |
| OncoTree Code | OCSC |
DepMap Information
| Source Type | Sigma-Aldrich |
|---|---|
| Source ID | ACH-001509_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Arg110Cys (c.328C>T) | Heterozygous | - | PubMed=29970484 |
| MutationSimple | HRAS | p.Gly13Ser (c.37G>A) | Heterozygous | - | Unknown |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X,Y
CSF1PO
11,13
D13S317
12,13
D16S539
11,12
D18S51
14,17
D21S11
29,31.2
D3S1358
17,18
D5S818
12,14
D7S820
11
D8S1179
13
FGA
23,26
Penta D
6,12
Penta E
11,13
TH01
6,9
TPOX
8,11
vWA
17,19
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Development and characterization of in vitro human oral mucosal equivalents derived from immortalized oral keratinocytes.
Trivedi H.M., Murdoch C., Whawell S.A.
Tissue Eng. Part C. Methods 22:1108-1117(2016).
Normal and malignant epithelial cells with stem-like properties have an extended G2 cell cycle phase that is associated with apoptotic resistance.
Mackenzie I.C.
BMC Cancer 10:166.1-166.16(2010).
Stem cell patterns in cell lines derived from head and neck squamous cell carcinoma.
Harper L.J., Piper K., Common J.E.A., Fortune F., Mackenzie I.C.
J. Oral Pathol. Med. 36:594-603(2007).
Restoration of alpha V beta 5 integrin expression in neoplastic keratinocytes results in increased capacity for terminal differentiation and suppression of anchorage-independent growth.
Jones J., Sugiyama M., Speight P.M., Watt F.M.
Oncogene 12:119-126(1996).
Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
Yeudall W.A., Paterson I.C., Patel V., Prime S.S.
Eur. J. Cancer B. Oral Oncol. 31:136-143(1995).