MM253Homo sapiens (Human)Cancer cell line

Also known as: MM 253

🤖 AI SummaryBased on 6 publications

Quick Overview

Human melanoma cell line with BRAF and NRAS mutations

Detailed Summary

MM253 is a human melanoma cell line derived from a melanoma tumor. It is characterized by specific genetic mutations in the BRAF and NRAS genes, which are commonly associated with melanoma development. These mutations may influence the cell line's response to targeted therapies. The cell line has been used in studies to identify gene expression signatures and mutation profiles relevant to melanoma, contributing to the understanding of molecular mechanisms in melanoma progression and treatment strategies.

Research Applications

Identification of BRAF and NRAS mutation profilesGene expression signature analysis for melanomaMutation detection and validation in melanoma cell lines

Key Characteristics

BRAF mutation (V600E)NRAS mutation (Q61L)Used in studies of melanoma molecular mechanisms

Generated on 6/19/2025

Basic Information

Database ID	CVCL_2604
Species	Homo sapiens (Human)
Tissue Source	Lymph node[UBERON:UBERON_0000029]

Donor Information

Age	46
Age Category	Adult
Sex	Male

Disease Information

Disease	Amelanotic melanoma
Lineage	Skin
Subtype	Melanoma
OncoTree Code	MEL

DepMap Information

Source Type	CellBank Australia
Source ID	ACH-001970_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	BRAF	p.Val600Glu (c.1799T>A)	Unspecified	-	PubMed=26214590
Gene deletion	CDKN2A	-	Homozygous	Possible	PubMed=26870271

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

10,11

D13S317

11,12

D16S539

D18S51

18,19

D19S433

D21S11

D2S1338

16,20

D3S1358

D5S818

11,12

D7S820

8,10

D8S1179

11,14

FGA

Penta D

Penta E

9,10

TH01

9.3

TPOX

8,11

vWA

16,18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

A high-throughput panel for identifying clinically relevant mutation profiles in melanoma.

Scolyer R.A., Mann G.J., Schmidt C.W., Herington A., Hayward N.K.

Mol. Cancer Ther. 11:888-897(2012).

DOI PubMed

Confirmation of a BRAF mutation-associated gene expression signature in melanoma.

Johansson P., Pavey S., Hayward N.K.

Pigment Cell Res. 20:216-221(2007).

DOI PubMed

Genome-wide loss of heterozygosity and copy number analysis in melanoma using high-density single-nucleotide polymorphism arrays.

Stark M.S., Hayward N.K.

Cancer Res. 67:2632-2642(2007).

DOI PubMed

Microarray expression profiling in melanoma reveals a BRAF mutation signature.

Meltzer P.S., Ringner M., Hayward N.K.

Oncogene 23:4060-4067(2004).

DOI PubMed

p53 mutations in human cutaneous melanoma correlate with sun exposure but are not always involved in melanomagenesis.

Zerp S.F., van Elsas A., Peltenburg L.T.C., Schrier P.I.

Br. J. Cancer 79:921-926(1999).

DOI PubMed PMC

CDKN2A/p16 is inactivated in most melanoma cell lines.";

Gabrielli B.G., Parsons P.G., Hayward N.K.

Cancer Res. 57:4868-4875(1997).

PubMed

Properties of human melanoma cells resistant to 5-(3',3'-dimethyl-1-triazeno)imidazole-4-carboxamide and other methylating agents.

Parsons P.G., Smellie S.G., Morrison L.E., Hayward I.P.

Cancer Res. 42:1454-1461(1982).

PubMed

Effects of calcium depletion on human cells in vitro and the anomalous behavior of the human melanoma cell line MM170.

Parsons P.G., Musk P., Goss P.D., Leah J.

Cancer Res. 43:2081-2087(1983).

PubMed

The effect of hyperthermia and melphalan on survival of human fibroblast strains and melanoma cell lines.

Goss P.D., Parsons P.G.

Cancer Res. 37:152-156(1977).

PubMed

Human malignant melanoma cell lines.";

Pope J.H., Morrison L.E., Moss D.J., Parsons P.G., Dunne R.M.

Pathology 11:191-195(1979).

DOI PubMed

A cytogenetic study of eight human melanoma cell lines.";

Muir P.D., Gunz F.W.

Pathology 11:597-606(1979).

DOI PubMed