EJ-1Homo sapiens (Human)Cancer cell line
Also known as: EJ1
🤖 AI SummaryBased on 3 publications
Quick Overview
EJ-1 cell line for cancer research and drug testing.
Detailed Summary
EJ-1 is a human cell line derived from bladder carcinoma. It is widely used in cancer research, particularly for studying bladder cancer. The cell line has been identified as being cross-contaminated with T24 cells, which may affect its reliability as a model system. STR profiling has been used to confirm the identity of EJ-1 and to detect cross-contamination with other cell lines. This cell line is important for understanding the genetic and molecular characteristics of bladder cancer and for developing targeted therapies.
Research Applications
Cancer researchDrug testingCell line authentication
Key Characteristics
Cross-contaminated with T24 cellsUsed for bladder cancer studies
Generated on 6/19/2025
Basic Information
| Database ID | CVCL_2893 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Urinary bladder[UBERON:UBERON_0001255] |
Donor Information
| Age | 82 |
|---|---|
| Age Category | Adult |
| Sex | Female |
Disease Information
| Disease | Bladder carcinoma |
|---|---|
| Lineage | Lymphoid |
| Subtype | Diffuse Large B-Cell Lymphoma, NOS |
| OncoTree Code | DLBCLNOS |
DepMap Information
| Source Type | ATCC |
|---|---|
| Source ID | ACH-003100_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Tyr126Ter (c.378C>G) | Homozygous | - | from parent cell line T24 |
| MutationSimple | TERT | c.1-124C>T (c.228C>T) (C228T) | Unspecified | In promoter | from parent cell line Hep-G2 |
| MutationSimple | HRAS | p.Gly12Val (c.35G>T) | Homozygous | - | from parent cell line T24 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
10,12
D13S317
12
D16S539
9
D18S51
16,18
D21S11
29
D5S818
10,12
D7S820
10,11
D8S1179
14
FGA
17,22
TH01
6
TPOX
8,11
vWA
17
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Freshney R.I.
Int. J. Cancer 127:1-8(2010).
Short tandem repeat profiling provides an international reference standard for human cell lines.
Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
Screening the p53 status of human cell lines using a yeast functional assay.
Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Mol. Carcinog. 19:243-253(1997).
Cell line individualization by STR multiplex system in the cell bank found cross-contamination between ECV304 and EJ-1/T24.
Mizusawa H.
Tissue Cult. Res. Commun. 18:329-338(1999).