HuH-28Homo sapiens (Human)Cancer cell line

Also known as: HUH28, HuH28, HUH-28

🤖 AI SummaryBased on 6 publications

Quick Overview

Human cholangiocellular carcinoma cell line with unique drug sensitivity profiles.

Detailed Summary

HuH-28 is a human cholangiocellular carcinoma (CCC) cell line established from a liver tumor. It exhibits slow growth with a doubling time of approximately 80 hours and has been passaged over 20 times. The cell line shows spindle and polygonal morphology and expresses tumor markers such as alkaline phosphatase (ALP), gamma-glutamyltranspeptidase (GGT), and ferritin. It does not form tumors in nude mice but secretes various markers. Research indicates its potential for studying carcinogenesis and drug resistance mechanisms, particularly in relation to gemcitabine sensitivity. The cell line's genetic characteristics include hypotriploid chromosome numbers and specific marker chromosomes, suggesting its utility in cancer research.

Research Applications

Carcinogenesis studiesDrug sensitivity profilingTumor marker analysis

Key Characteristics

Slow growth rateTumor marker secretionHypotriploid chromosome number

Generated on 6/19/2025

Basic Information

Database ID	CVCL_2955
Species	Homo sapiens (Human)
Tissue Source	Liver[UBERON:UBERON_0002107]

Donor Information

Age	37
Age Category	Adult
Sex	Female
Race	asian

Disease Information

Disease	Cholangiocarcinoma
Lineage	Biliary Tract
Subtype	Intrahepatic Cholangiocarcinoma
OncoTree Code	IHCH

DepMap Information

Source Type	HSRRB
Source ID	ACH-000808_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Glu271Lys (c.811G>A)	Heterozygous	-	Unknown
MutationSimple	PIK3CA	p.Glu545Lys (c.1633G>A)	Heterozygous	-	from parent cell line MCF-7

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

9,12

D13S317

9,12

D16S539

D18S51

D19S433

13,14.2

D21S11

30,33.2

D2S1338

D3S1358

15,16

D5S818

9,12

D7S820

D8S1179

FGA

Penta D

9,10

Penta E

13,22

TH01

TPOX

vWA

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Generation of a biliary tract cancer cell line atlas reveals molecular subtypes and therapeutic targets.

Vazquez F., Getz G., Bardeesy N.M.

bioRxiv 2024:07.04.601970-07.04.601970(2024).

DOI PubMed PMC

Dependency of cholangiocarcinoma on cyclin D-dependent kinase activity.

Jirawatnotai S.

Hepatology 70:1614-1630(2019).

DOI PubMed

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma.

Bardeesy N.M.

Cancer Discov. 6:727-739(2016).

DOI PubMed PMC

Antitumor effect of the novel sphingosine kinase 2 inhibitor ABC294640 is enhanced by inhibition of autophagy and by sorafenib in human cholangiocarcinoma cells.

Zou X.-P., Thomas M.B., Smith C.D., Roberts L.R.

Oncotarget 7:20080-20092(2016).

DOI PubMed PMC

A resource for cell line authentication, annotation and quality control.

Neve R.M.

Nature 520:307-311(2015).

DOI PubMed

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

Nature 483:603-607(2012).

DOI PubMed PMC

Gene expression analysis for predicting gemcitabine resistance in human cholangiocarcinoma.

Tsuchiya T., Gotoh M.

J. Hepatobiliary Pancreat. Sci. 18:700-711(2011).

DOI PubMed

Signatures of mutation and selection in the cancer genome.";

Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Nature 463:893-898(2010).

DOI PubMed PMC

Screening the p53 status of human cell lines using a yeast functional assay.

Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.

Mol. Carcinog. 19:243-253(1997).

DOI PubMed

Establishment and characterization of a human cholangiocellular carcinoma cell line.

Kusaka Y., Muraoka A., Tokiwa T., Sato J.

Hum. Cell 1:92-94(1988).

PubMed

Establishment and characterization of a cell line from a human cholangiocellular carcinoma.

Kusaka Y., Tokiwa T., Sato J.

Res. Exp. Med. (Berl.) 188:367-375(1988).

DOI PubMed