THLE-3Homo sapiens (Human)Transformed cell line
Also known as: Transformed Human Liver Epithelial-3, THLE3
🤖 AI SummaryBased on 3 publications
Quick Overview
Immortalized human liver epithelial cells for toxicology and cancer research.
Detailed Summary
THLE-3 is an immortalized human liver epithelial cell line derived from normal liver tissue. These cells retain key hepatocyte characteristics, including the expression of albumin and cytokeratin 18, and are capable of metabolizing chemical carcinogens such as aflatoxin B1, benzo[a]pyrene, and N-nitrosodimethylamine. THLE-3 cells are nontumorigenic when injected into athymic nude mice and exhibit near-diploid karyotypes. They are widely used in studies related to xenobiotic metabolism, carcinogen activation, and liver toxicity. The cell line has been utilized in research on telomerase activity, p53 mutations, and the role of SV40 T antigen in immortalization. THLE-3 provides a valuable in vitro model for investigating the molecular mechanisms of liver carcinogenesis and drug metabolism.
Research Applications
Xenobiotic metabolism studiesCarcinogen activation and DNA adduct formationLiver toxicity testingTelomerase activity analysisp53 mutation studiesSV40 T antigen immortalization mechanisms
Key Characteristics
Nontumorigenic in vivoNear-diploid karyotypeExpression of hepatocyte markers (albumin, cytokeratin 18)Metabolism of multiple carcinogensImmortalized via SV40 T antigenHigh passage viability
Generated on 6/20/2025
Basic Information
| Database ID | CVCL_3804 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Liver[UBERON:UBERON_0002107] |
Donor Information
| Age Category | Adult |
|---|---|
| Sex | Unknown |
Disease Information
| Disease | Non-Cancerous |
|---|---|
| Lineage | Fibroblast |
| Subtype | Fibroblast, Soft Tissue |
DepMap Information
| Source Type | ATCC |
|---|---|
| Source ID | ACH-003308_source |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
11,12
D13S317
13
D16S539
11,12
D5S818
13
D7S820
8,10
TH01
8,9.3
TPOX
6,9
vWA
17,18
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J.
Nat. Commun. 4:2218.1-2218.7(2013).
p53 mutations in human immortalized epithelial cell lines.";
Harris C.C.
Carcinogenesis 14:833-839(1993).
Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.
Lechner J.F., Harris C.C.
Proc. Natl. Acad. Sci. U.S.A. 90:5123-5127(1993).
A non-tumorigenic human liver epithelial cell culture model for chemical and biological carcinogenesis investigations.
Groopman J.D., Shields P.G., Tokiwa T., Harris C.C.
(In book chapter) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.307-321; Humana Press; New York; USA (1990).