LN-215Homo sapiens (Human)Cancer cell line
Also known as: LN215, LN 215
🤖 AI SummaryBased on 6 publications
Quick Overview
Human glioblastoma cell line with astrocytic features, used in cancer research.
Detailed Summary
LN-215 is a human glioblastoma cell line derived from a malignant astrocytoma. It exhibits glial morphology with polygonal cells forming a honeycomb-like structure. The cell line expresses glial fibrillary acidic protein (GFAP) and shows high levels of 2'3' cyclic nucleotide 3' phosphohydrolase (CNPase) activity. LN-215 has been used in studies investigating the role of MNK1 in regulating SMAD2-dependent TGF-β signaling pathways in glioblastoma. It is also utilized in research on genetic alterations, including mutations in TP53, PTEN, and p16/ARF, and in studies examining cell line authenticity through STR profiling. LN-215 is known for its tumorigenic potential in immunocompromised mice and is a valuable tool for studying glioma biology and therapeutic strategies.
Research Applications
Cancer researchGenetic alteration studiesTumor biologyTherapeutic strategy development
Key Characteristics
Expresses GFAPHigh CNPase activityTumorigenic in nude miceMutations in TP53, PTEN, and p16/ARFUsed in MNK1 and TGF-β signaling studies
Generated on 6/20/2025
Basic Information
| Database ID | CVCL_3954 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Brain, temporo-occipital lobe |
Donor Information
| Age | 60 |
|---|---|
| Age Category | Adult |
| Sex | Female |
Disease Information
| Disease | Astrocytoma |
|---|---|
| Lineage | CNS/Brain |
| Subtype | Glioblastoma |
| OncoTree Code | GB |
DepMap Information
| Source Type | Academic lab |
|---|---|
| Source ID | ACH-000328_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.Pro191_Gln192del | Heterozygous | - | Unknown, PubMed=10416987 |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
10,11
D13S317
14
D16S539
9,12
D18S51
14,16
D21S11
29
D3S1358
14,17
D5S818
12
D7S820
10
D8S1179
10,14
FGA
22,25
Penta D
9,13
Penta E
7,11
TH01
8
TPOX
8
vWA
18
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Sellers W.R.
Nature 569:503-508(2019).
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Golub T.R., Root D.E., Hahn W.C.
Sci. Data 1:140035-140035(2014).
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Hamou M.-F., Delorenzi M., Hegi M.E.
Neuro-oncol. 14:701-711(2012).
MAP kinase-interacting kinase 1 regulates SMAD2-dependent TGF-beta signaling pathway in human glioblastoma.
Moncayo G., Hemmings B.A.
Cancer Res. 71:2392-2402(2011).
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Van Meir E.G.
Brain Pathol. 9:469-479(1999).
The human melanoma antigen-encoding gene, MAGE-1, is expressed by other tumour cells of neuroectodermal origin such as glioblastomas and neuroblastomas.
Rimoldi D., Romero P., Carrel S.
Int. J. Cancer 54:527-528(1993).
Variant CD44 adhesion molecules are expressed in human brain metastases but not in glioblastomas.
Diserens A.-C., Van Meir E.G.
Cancer Res. 53:5345-5349(1993).