LN-319Homo sapiens (Human)Cancer cell line

Also known as: LN319, LN 319

🤖 AI SummaryBased on 9 publications

Quick Overview

Human glioblastoma cell line with p53 mutations and resistance to Apo2L/TRAIL.

Detailed Summary

LN-319 is a human glioblastoma cell line derived from a malignant astrocytoma. It exhibits mutations in the TP53 gene, specifically a codon 175 (Arg → His) mutation, and alterations in the PTEN and p16/ARF genes. The cell line is known for its resistance to Apo2L/TRAIL-induced apoptosis, which may be linked to low expression of DR5 receptors. LN-319 is commonly used in studies investigating the mechanisms of drug resistance and apoptosis in glioblastoma. It has been utilized in research on the role of p53 in tumor suppression and in evaluating the efficacy of targeted therapies. The cell line is also part of panels for assessing genetic diversity and ancestry in cancer research.

Research Applications

Apoptosis and drug resistance studiesp53 mutation analysisGenetic diversity and ancestry researchTargeted therapy evaluation

Key Characteristics

TP53 mutation (Arg175His)PTEN and p16/ARF alterationsResistance to Apo2L/TRAILLow DR5 expression

Generated on 6/20/2025

Basic Information

Database ID	CVCL_3958
Species	Homo sapiens (Human)
Tissue Source	Brain, right temporal lobe[UBERON:UBERON_0002809]

Donor Information

Age	67
Age Category	Adult
Sex	Male

Disease Information

Disease	Astrocytoma
Lineage	CNS/Brain
Subtype	Astrocytoma
OncoTree Code	ASTR

DepMap Information

Source Type	Academic lab
Source ID	ACH-000128_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	TP53	p.Arg175His (c.524G>A)	Unspecified	Somatic mutation acquired during proliferation	from parent cell line YCC-3
MutationSimple	PTEN	p.Arg15Ile (c.44G>T)	Homozygous	-	Unknown

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

X,Y

CSF1PO

D13S317

D16S539

11,12

D18S51

14,19

D21S11

30,31

D3S1358

16,17

D5S818

D7S820

D8S1179

FGA

19,26

Penta D

9,13

Penta E

15,17

TH01

9,9.3

TPOX

8,12

vWA

15,18

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Sellers W.R.

Nature 569:503-508(2019).

DOI PubMed PMC

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

Cancer Res. 79:1263-1273(2019).

DOI PubMed PMC

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Golub T.R., Root D.E., Hahn W.C.

Sci. Data 1:140035-140035(2014).

DOI PubMed PMC

DNA fingerprinting of glioma cell lines and considerations on similarity measurements.

Hamou M.-F., Delorenzi M., Hegi M.E.

Neuro-oncol. 14:701-711(2012).

DOI PubMed PMC

MAP kinase-interacting kinase 1 regulates SMAD2-dependent TGF-beta signaling pathway in human glioblastoma.

Moncayo G., Hemmings B.A.

Cancer Res. 71:2392-2402(2011).

DOI PubMed

Mechanisms of resistance of human glioma cells to Apo2 ligand/TNF-related apoptosis-inducing ligand.

Rieger J., Frank B., Weller M., Wick W.

Cell. Physiol. Biochem. 20:23-34(2007).

DOI PubMed

P-glycoprotein and multidrug resistance-associated protein mediate specific patterns of multidrug resistance in malignant glioma cell lines, but not in primary glioma cells.

Bahr O., Rieger J., Duffner F., Meyermann R., Weller M., Wick W.

Brain Pathol. 13:482-494(2003).

DOI PubMed PMC

CP-31398, a novel p53-stabilizing agent, induces p53-dependent and p53-independent glioma cell death.

Wischhusen J., Naumann U., Ohgaki H., Rastinejad F., Weller M.

Oncogene 22:8233-8245(2003).

DOI PubMed

Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.

Van Meir E.G.

Brain Pathol. 9:469-479(1999).

DOI PubMed PMC

Predicting chemoresistance in human malignant glioma cells: the role of molecular genetic analyses.

Krajewski S., Reed J.C., von Deimling A., Dichgans J.

Int. J. Cancer 79:640-644(1998).

DOI PubMed

A simple p53 functional assay for screening cell lines, blood, and tumors.

Tada M., Van Meir E.G., Estreicher A., Iggo R.D.

Proc. Natl. Acad. Sci. U.S.A. 92:3963-3967(1995).

DOI PubMed PMC

Variant CD44 adhesion molecules are expressed in human brain metastases but not in glioblastomas.

Diserens A.-C., Van Meir E.G.

Cancer Res. 53:5345-5349(1993).

PubMed

Web Resources

iclac.org