LN-443Homo sapiens (Human)Cancer cell line
Also known as: LN443, LN 443
🤖 AI SummaryBased on 6 publications
Quick Overview
Human glioblastoma cell line with p53, p16, and PTEN mutations.
Detailed Summary
LN-443 is a human glioblastoma cell line derived from a male patient with a median age of 66 years. It exhibits mutations in the TP53, p16, and PTEN genes, which are frequently altered in glioblastomas. These genetic alterations suggest a role in the development and progression of glioblastomas. The cell line is used in research to study the molecular mechanisms of glioma biology and to evaluate therapeutic strategies targeting these genetic alterations. LN-443 has been characterized for its tumorigenic potential and is part of a panel of glioma cell lines used to investigate the genetic diversity and functional consequences of these mutations.
Research Applications
Genetic analysis of glioblastoma mutationsTumorigenicity studiesTherapeutic target evaluation
Key Characteristics
TP53 mutationp16 deletionPTEN mutation
Generated on 6/20/2025
Basic Information
| Database ID | CVCL_3960 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Brain, left parietal lobe[UBERON:UBERON_0002802] |
Donor Information
| Age Category | Unknown |
|---|---|
| Sex | Female |
Disease Information
| Disease | Glioblastoma |
|---|---|
| Lineage | CNS/Brain |
| Subtype | Glioblastoma |
| OncoTree Code | GB |
DepMap Information
| Source Type | Academic lab |
|---|---|
| Source ID | ACH-000673_source |
Known Sequence Variations
| Type | Gene/Protein | Description | Zygosity | Note | Source |
|---|---|---|---|---|---|
| MutationSimple | TP53 | p.His214Arg (c.641A>G) | Unspecified | - | Unknown |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
10,12
D13S317
8
D16S539
10,11
D18S51
15,16
D21S11
28,30
D3S1358
15,17
D5S818
9,12
D7S820
10
D8S1179
13,14
FGA
21,23
Penta D
11,12
Penta E
7,13
TH01
7,9
TPOX
8
vWA
18,19
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Sellers W.R.
Nature 569:503-508(2019).
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
Cancer Res. 79:1263-1273(2019).
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Golub T.R., Root D.E., Hahn W.C.
Sci. Data 1:140035-140035(2014).
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Hamou M.-F., Delorenzi M., Hegi M.E.
Neuro-oncol. 14:701-711(2012).
Effect of aberrant p53 function on temozolomide sensitivity of glioma cell lines and brain tumor initiating cells from glioblastoma.
Cairncross J.G.
J. Neurooncol. 102:1-7(2011).
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Van Meir E.G.
Brain Pathol. 9:469-479(1999).
The human melanoma antigen-encoding gene, MAGE-1, is expressed by other tumour cells of neuroectodermal origin such as glioblastomas and neuroblastomas.
Rimoldi D., Romero P., Carrel S.
Int. J. Cancer 54:527-528(1993).
Variant CD44 adhesion molecules are expressed in human brain metastases but not in glioblastomas.
Diserens A.-C., Van Meir E.G.
Cancer Res. 53:5345-5349(1993).