KKU-100Homo sapiens (Human)Cancer cell line

Also known as: Khon Kaen University-100, K100, KKU100

🤖 AI SummaryBased on 2 publications

Quick Overview

Human cholangiocarcinoma cell line associated with liver fluke infection, used in cancer research.

Detailed Summary

KKU-100 is a human cholangiocarcinoma cell line derived from patients in the endemic area of liver fluke infection in Thailand. It is utilized in research to study the molecular mechanisms of cholangiocarcinoma, particularly in relation to Opisthorchis viverrini infection. The cell line has been characterized for its protein expression profiles, including the up-regulation of annexin A2 (ANXA2), which has been implicated in tumor progression and prognosis. Research involving KKU-100 has focused on identifying potential biomarkers and therapeutic targets for cholangiocarcinoma, with findings suggesting ANXA2 as a significant prognostic marker. The cell line is also used to investigate the role of genetic mutations, such as those in TP53 and KRAS, in cancer development and progression.

Research Applications

Cancer researchMolecular mechanisms of cholangiocarcinomaBiomarker discoveryTherapeutic target identification

Key Characteristics

Up-regulation of annexin A2 (ANXA2)Expression of TP53 and KRAS mutationsUse in studying liver fluke-associated carcinogenesis

Generated on 6/20/2025

Basic Information

Database ID	CVCL_3996
Species	Homo sapiens (Human)

Donor Information

Age	65
Age Category	Adult
Sex	Female

Disease Information

Disease	Klatskin tumor
Lineage	Biliary Tract
Subtype	Extrahepatic Cholangiocarcinoma
OncoTree Code	EHCH

DepMap Information

Source Type	JCRB
Source ID	ACH-001536_source

Known Sequence Variations

Type	Gene/Protein	Description	Zygosity	Note	Source
MutationSimple	KRAS	p.Gly12Asp (c.35G>A)	Unspecified	-	PubMed=29786757

Haplotype Information (STR Profile)

Short Tandem Repeat (STR) profile for cell line authentication.

Amelogenin

CSF1PO

11,13

D13S317

D16S539

10,11

D18S51

D21S11

31.2,33.2

D3S1358

16,17

D5S818

11,13

D7S820

8,11

D8S1179

10,15

FGA

22,25

Penta D

Penta E

9,11

TH01

8,9

TPOX

8,9

vWA

17,19

Gene Expression Profile

Gene expression levels and statistical distribution

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Full DepMap dataset with combined data across cell lines

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Publications

Generation of a biliary tract cancer cell line atlas reveals molecular subtypes and therapeutic targets.

Vazquez F., Getz G., Bardeesy N.M.

bioRxiv 2024:07.04.601970-07.04.601970(2024).

DOI PubMed PMC

Development and characterization of human primary cholangiocarcinoma cell lines.

Glaser S., Kennedy L., Francis H., Zhang W.-J., Alpini G.D., Ekser B.

Am. J. Pathol. 192:1200-1217(2022).

DOI PubMed PMC

Dependency of cholangiocarcinoma on cyclin D-dependent kinase activity.

Jirawatnotai S.

Hepatology 70:1614-1630(2019).

DOI PubMed

Establishment of cholangiocarcinoma cell lines from patients in the endemic area of liver fluke infection in Thailand.

Kukongviriyapan V.

Tumor Biol. 39:1010428317725925-1010428317725925(2017).

DOI PubMed

Cyclophilin A enhances cell proliferation and tumor growth of liver fluke-associated cholangiocarcinoma.

Yao Q.-Z., Chen C.-Y.

Mol. Cancer 10:102.1-102.15(2011).

DOI PubMed PMC

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker.

Sripa B.

Int. J. Parasitol. 40:1203-1212(2010).

DOI PubMed PMC

Triptolide sensitizes resistant cholangiocarcinoma cells to TRAIL-induced apoptosis.

Panichakul T., Intachote P., Wongkajornsilp A., Sripa B., Sirisinha S.

Anticancer Res. 26:259-265(2006).

PubMed

Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (KKU-100).

Bhudhisawasdi V., Puapairoj A., Sripa C., Miwa M.

World J. Gastroenterol. 11:3392-3397(2005).

DOI PubMed PMC

Drug sensitivity and drug resistance profiles of human intrahepatic cholangiocarcinoma cell lines.

Bhudhisawasdi V., Tassaneeyakul W.

World J. Gastroenterol. 11:2748-2753(2005).

DOI PubMed PMC

Synergistic cytotoxicity and apoptosis induced in human cholangiocarcinoma cell lines by a combined treatment with tumor necrosis factor-alpha (TNF-alpha) and triptolide.

Panichakul T., Wanun T., Reutrakul V., Sirisinha S.

Asian Pac. J. Allergy Immunol. 20:167-173(2002).

PubMed