SSP-25Homo sapiens (Human)Cancer cell line
Also known as: SSP25
🤖 AI SummaryBased on 3 publications
Quick Overview
Human intrahepatic cholangiocarcinoma cell line with unique drug sensitivity profile.
Detailed Summary
SSP-25 is a human intrahepatic cholangiocarcinoma cell line established from a patient with sarcomatous change in their tumor. The cell line exhibits distinct morphological characteristics, including spindle-shaped cells with intracytoplasmic vacuoles. SSP-25 shows differential expression of marker proteins compared to its adenocarcinoma counterpart, RBE, with absence of carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9). Research indicates that SSP-25 has a unique drug sensitivity profile, with specific responses to chemotherapeutic agents. The cell line has been used in studies examining the molecular mechanisms of cholangiocarcinoma progression and drug resistance. Additionally, SSP-25 has been utilized in in vitro studies to investigate the potential for sarcomatoid cells to differentiate into more mature adenocarcinoma cells, suggesting a possible role of cytokines in this transformation process.
Research Applications
Drug sensitivity profilingMolecular mechanisms of cholangiocarcinoma progressionIn vitro differentiation studiesCytokine-mediated transformation studies
Key Characteristics
Spindle-shaped morphology with intracytoplasmic vacuolesAbsence of CEA and CA19-9 expressionUnique drug response profilePotential for differentiation into adenocarcinoma cells
Generated on 6/21/2025
Basic Information
| Database ID | CVCL_4902 |
|---|---|
| Species | Homo sapiens (Human) |
| Tissue Source | Ascites[UBERON:UBERON_0007795] |
Donor Information
| Age | 61 |
|---|---|
| Age Category | Adult |
| Sex | Female |
Disease Information
| Disease | Cholangiocarcinoma |
|---|---|
| Lineage | Biliary Tract |
| Subtype | Intrahepatic Cholangiocarcinoma |
| OncoTree Code | IHCH |
DepMap Information
| Source Type | Academic lab |
|---|---|
| Source ID | ACH-001858_source |
Haplotype Information (STR Profile)
Short Tandem Repeat (STR) profile for cell line authentication.
Amelogenin
X
CSF1PO
12
D13S317
12
D16S539
10,12
D18S51
21
D21S11
29
D3S1358
15
D5S818
10
D7S820
11
D8S1179
10,12
FGA
19,25
Penta D
14
Penta E
15
TH01
7,9.3
TPOX
8
vWA
14,17
Gene Expression Profile
Gene expression levels and statistical distribution
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Full DepMap dataset with combined data across cell lines
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Publications
Generation of a biliary tract cancer cell line atlas reveals molecular subtypes and therapeutic targets.
Vazquez F., Getz G., Bardeesy N.M.
bioRxiv 2024:07.04.601970-07.04.601970(2024).
Development and characterization of human primary cholangiocarcinoma cell lines.
Glaser S., Kennedy L., Francis H., Zhang W.-J., Alpini G.D., Ekser B.
Am. J. Pathol. 192:1200-1217(2022).
Dependency of cholangiocarcinoma on cyclin D-dependent kinase activity.
Jirawatnotai S.
Hepatology 70:1614-1630(2019).
Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma.
Bardeesy N.M.
Cancer Discov. 6:727-739(2016).
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Yamori T.
Mol. Cancer Ther. 4:399-412(2005).